Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells

Figure 4

Effects of pretreatment with AL4W-1b extract on proinflammatory cytokines in RAW264 cells. Amounts of (a) IL-1β and (b) IL-6 production in RAW264 cells are shown. Cells were pretreated with or without AL4W-1b extract at concentrations of 1/5000 and 1/1000 for 24 h. After treatment, cells were activated with LPS (1 ng/mL) for 12 h. The secreted proinflammatory mediators were determined by ELISA assay with the MAGPIX xPONENT system. Values are expressed as the mean ± SD of duplicate experiments. A mean value that is significantly different from that of the control LPS (+) group is indicated as p < 0.01.

(a)

(b)