Research Article
Melatonin Attenuates β-Glycerophosphate-Induced Calcification of Vascular Smooth Muscle Cells via a Wnt1/β-Catenin Signaling Pathway
Figure 3
Effects of melatonin and lithium chloride (LiCl, 5 mM) on β-GP-induced calcium deposition in VSMCs (n = 3). (a) Result of Alizarin Red Staining. (b) Result of calcium concentration. (c) Result of ALP level. (d) Result of interleukin-1β (IL-1β) level. (e-g) Results of α-SMA, Runx2, and cleaved caspase-3 protein expression. versus Con, versus β-GP, and versus β-GP + Mel.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |