Insulin-Like Growth Factor Binding Protein 5—A Probable Target of Kidney Renal Papillary Renal Cell Carcinoma

Figure 5

Expression of IGFBP5 in Caki-2 cell line. (a) Expression of VEGFA after inhibited by siRNA (n = 3). The fold change is 3.1 (). (b) Expression of TGF-β after inhibited by siRNA (n = 3). The fold change is 5.4 (). (c) Expression of IGFBP5 after VEGFA and TGF-β blocked by siRNA (n = 3). IGFBP5 was downregulated by 2.6-fold and 4.0 fold respectively (). (d) Expression of IGFBP5 after stimulated by VEGF₁₆₅ and TGF-β (n = 3). IGFBP5 was upregulated by 2.8-fold and 6.2-fold respectively (). (e) Cell proliferation rate of Caki-2 detected by MTT assay (n = 3). The proliferation rate of IGFBP5 stimulation group is higher than normal control group (), and cell proliferation of IGFBP5 knockdown group is lower than normal control group (). (f) Cell proliferation rate of Caki-2 detected by MTT assay (n = 3). The proliferation rate of VEGF₁₆₅ stimulation group is higher than normal control group (), and cell proliferation of TGF-β stimulation group is lower than normal control group (). (n  = 3) (*, ;**, ;***, ).

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