Bone Formation and Adaptive Morphology of the Anterior Tibial Muscle in 3-mm Daily Lengthening Using High-Fractional Automated Distraction and Osteosynthesis with the Ilizarov Apparatus Combined with Intramedullary Hydroxyapatite-Coated Wire
Figure 4
Ninety days after removal of the apparatus. Muscle histostructure in group 1 (a, b, c) and group 2 (d, e, f): (a) muscle structure approximated to normal; (b) normal striation of muscle fiber; (с) unchanged vessels of arterial and venous types in the intact perimysium; (d) variability of muscle fiber diameters, with increased portion of white fibers maintained (type 1); (e) preservation of increased number of fibroblasts in the endomysium; (f) normal muscle structure in an intact dog for comparison. Transverse cryostat sections, myosin ATPase reaction at pH 9.0 (Padykula and Herman). Magnification: 16x (lens), 12.5х (eyepiece) (a, d, f). Semithin sections, stained by M. Ontell. Magnification: 100x (lens), 12.5x (eyepiece) (b, e). Paraffin section, stained with hematoxylin and eosin; magnification: (с) 16x (lens), 12.5x (eyepiece).