BioMed Research International / 2019 / Article / Tab 2

Research Article

Technical Verification and Assessment of Independent Validation of Biomarker Models for Endometriosis

Table 2

Overview of immunological assays used in study by Vodolazkaia et al. and in the current technical verification and validation studies.

ProteinAssay in original study [11]Assay in technical verification study (collaboration with Roche Diagnostics)Assay in validation study (in-house at KU Leuven)Use of the same assay between studies

CA-125Roche Modular E170 
Roche Diagnostics GmbH
cobas e 601 
Roche Diagnostics GmbH
Roche Modular E170 
Roche Diagnostics GmbH
Yes (successor system)

VEGF-ABioplex multiplex immunoassay, 
BioRad Laboratories, Hercules, USA
cobas e 601 
Roche Diagnostics GmbH (Internal Research Assay)
ELISA 
Cloud-clone corp, Houston, USA
No

Annexin-VELISA 
American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)
ELISA 
American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)
ELISA 
American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)
Yes

GlycodelinELISA 
Bioserv Diagnostics, Rostock, Germany
ELISA 
Roche Diagnostics GmbH (Internal Research Assay)
ELISA 
Bioserv Diagnostics, Rostock, Germany
Only between Vodolazkaia et al. and validation study

sICAM-1ELISA 
R&D systems, Minneapolis, USA
IMPACT 
Roche Diagnostics GmbH (Internal Research Assay)
ELISA 
R&D systems, Minneapolis, USA
Only between Vodolazkaia et al. and validation study

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