Technical Verification and Assessment of Independent Validation of Biomarker Models for Endometriosis

Table 2

Overview of immunological assays used in study by Vodolazkaia et al. and in the current technical verification and validation studies.

Protein	Assay in original study [11]	Assay in technical verification study (collaboration with Roche Diagnostics)	Assay in validation study (in-house at KU Leuven)	Use of the same assay between studies
CA-125	Roche Modular E170; Roche Diagnostics GmbH	cobas e 601; Roche Diagnostics GmbH	Roche Modular E170; Roche Diagnostics GmbH	Yes (successor system)
VEGF-A	Bioplex multiplex immunoassay,; BioRad Laboratories, Hercules, USA	cobas e 601; Roche Diagnostics GmbH (Internal Research Assay)	ELISA; Cloud-clone corp, Houston, USA	No
Annexin-V	ELISA; American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)	ELISA; American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)	ELISA; American Diagnostica GmbH Pfungstadt, Germany (now Sekisui Diagnostics GmbH)	Yes
Glycodelin	ELISA; Bioserv Diagnostics, Rostock, Germany	ELISA; Roche Diagnostics GmbH (Internal Research Assay)	ELISA; Bioserv Diagnostics, Rostock, Germany	Only between Vodolazkaia et al. and validation study
sICAM-1	ELISA; R&D systems, Minneapolis, USA	IMPACT; Roche Diagnostics GmbH (Internal Research Assay)	ELISA; R&D systems, Minneapolis, USA	Only between Vodolazkaia et al. and validation study