A Rat Model of Radiation Vasculitis for the Study of Mesenchymal Stem Cell-Based Therapy

Figure 3

Histology and cytokine analysis of abdominal aortas. (a) Cross-sectional images of abdominal aorta. Serial cross sections of aorta were processed with hematoxylin-eosin stain (H&E), Masson’s trichrome stain (Masson), and immunostaining with antimyeloperoxidase antibody using DAB substrate kit (MPO), respectively. The images represented the investigation of eight rats for each group. Scar bar 100 μm. (b) Histological analysis of intimal hyperplasia. The relative intimal thickness was normalized to full thickness of vascular wall and expressed as a percentage. Eight rats were investigated for each group. Group comparison was performed with Mann–Whitney U test. < 0.05. (c) Expression of proinflammatory cytokines in aortas. The cytokine levels were measured by real-time qualitative reverse transcription PCR and normalized to vehicle control. The experiment was repeated three times for each group. Group comparison was performed with Mann–Whitney U test. < 0.05.