Eya2 Is Overexpressed in Human Prostate Cancer and Regulates Docetaxel Sensitivity and Mitochondrial Membrane Potential through AKT/Bcl-2 Signaling

Figure 3

Eya2 regulates docetaxel sensitivity and apoptosis. (a) PC-3 and LNCaP cells experiencing Eya2 transfection/shRNA knockdown were treated with different concentration of docetaxel (2.5, 5, 10, and 15 nM). CCK-8 assays were used to determine cell viability. The overexpression of Eya2 led to an increase in cell viability in both cell lines while the depletion of Eya2 led to downregulated cell viability. (b) PC-3 and LNCaP cells experiencing Eya2 transfection/shRNA knockdown were treated with 5nM docetaxel for 24 h. Annexin V/PI staining showed that Eya2 overexpression inhibited the docetaxel-induced rate of apoptosis. Eya2 shRNA increased the rate of docetaxel-induced apoptosis. p < 0.05.

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