Eya2 Is Overexpressed in Human Prostate Cancer and Regulates Docetaxel Sensitivity and Mitochondrial Membrane Potential through AKT/Bcl-2 Signaling
Figure 4
Eya2 regulates mitochondrial membrane potential and related proteins. (a) The overexpression of Eya2 inhibited the expression of cleaved-caspase3, cleaved-PARP and upregulated caspase3, PARP in docetaxel treated cells. The knockdown of Eya2 using shRNA led to an upregulation in the levels of cleaved-caspase3, cleaved-PARP and downregulated caspase3, PARP in docetaxel treated cells. (b) JC-1 staining was used to determine Δψm. JC-1 is normally visualized as green when Δψm is reduced. Our JC-1 staining results showed that the overexpression of Eya2 upregulated the Δψm (as shown by a reduction in the proportion of green staining) while Eya2 shRNA reduced Δψm (as shown by an increased proportion of green staining) after CDDP treatment. (c) Protein levels of Bcl-2, MMP7, p-AKT, and AKT in prostate cancer cells transfected with the Eya2 plasmid and shRNA; the overexpression of Eya2 increased the expression of Bcl-2, MMP7, and p-AKT proteins while the application of shRNA caused levels of these proteins to decrease. (d) Realtime PCR showed that the overexpression of Eya2 o increased the mRNA levels of Bcl-2 and MMP7 in prostate cancer cells while the application of shRNA caused a reduction in the expression of these proteins. p<0.05.