Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells

Figure 3

Role of ERK1/2 in uric acid-induced EMT in NRK-52E cells. (a) Protein expression of pERK and ERK by Western blot when exposed to different concentrations of UA. (b–e) Cells were treated by 600 mol/L UA with or without ERK1/2 pathway inhibitor PD98059 (10 mol/L) or UO126 (10 mol/L). Protein expression of pERK and ERK was detected by Western blot (b). ET-1 concentration in supernatant was measured by ELISA (c). mRNA expression of E-cadherin and α-SMA by RT-PCR (d). Protein expression of E-cadherin and α-SMA by Western blot (e). The results are presented as mean ± SD obtained from three independent experiments. P<0.05; P<0.01. NC, normal control; UA, uric acid.

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