Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells

Figure 4

Role of NOX4/ROS in uric acid-induced EMT in NRK-52E cells. (a) ROS production measured by DHE staining when exposed to different concentrations of UA. Magnification, ×200. (b–f) Cells were treated by 600 mol/L UA with or without classic antioxidant apocynin (20 mol/L) or DPI (10 mol/L). Level of ROS determined by DHE staining. Magnification, ×200 (b). Protein expression of pERK and ERK by Western blot (c). ET-1 concentration in supernatant by ELISA (d). mRNA expression of E-cadherin and α-SMA (e). Protein expression of E-cadherin and α-SMA (f). The results are presented as mean ± SD obtained from three independent experiments. P<0.05; P<0.01. NC, normal control; UA, uric acid; DHE, dihydroethidium; ELISA, enzyme linked immunosorbent assay.

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