Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells

Figure 5

Telmisartan prevented uric acid-induced EMT in NRK-52E cells. Cells were treated by 600 mol/L UA with or without telmisartan (10²–10⁴ nmol/L) for 48 h. (a) Expression of NOX4, E-cadherin, and α-SMA mRNA levels by RT-PCR. (b) Expression of NOX4, E-cadherin, α-SMA, and fibronectin protein levels by Western blot. Expression of E-cadherin (c) and α-SMA (d) protein level and cellular localization by immunofluorescence. Magnification, ×200. (e) The morphological changes of NRK-52E cells by microscopy in different groups. Scale bar = 20 m. The results are presented as mean ± SD obtained from three independent experiments. P<0.05; P<0.01. NC, normal control; UA, uric acid; TE, telmisartan.

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