SprI/SprR Quorum Sensing System of Serratia proteamaculans 94

Table 1

Strains and plasmids used in this study.

Strains or plasmids	Characteristics	Source or reference
Strains
Serratia proteamaculans 94	Isolated from spoiled refrigerated meat	Demidyuk et al. [10]
S. proteamaculans 94 rif-r	rif-r spontaneous mutant	Zaitseva et al. [16]
Escherichia coli S17-1 (λ-pir)	thi pro hsdR hsdM recA rpsL RP4-2 (Tc^r::Mu) (Km^r::Tn7) (λ-pir)	Simon et al. [17]
E. coli TG-1	K-12 supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5, (rK^-mK^-)	StrataGene
	F′ [tra D36 proAB^+lacI^qlac ZΔM15]
E. coli XL-1 blue	recA1 endA1 gyrA96 thi1 hsdR17 supE44 relA1 lac	Stratagene
Chromobacterium violaceum CV026	Violacein production-based AHL bioreporter, Km-r	McClean et al. [18]
Agrobacterium tumefaciens NT1/pZLR4	pZLR4 traG::lacZ β-galactosidase induction-based AHL bioreporter, Gm^r Cb^r	Shaw et al. [19]
S. proteamaculans 94 rif-r sprI::Gm	sprI::Gm gene replacement mutant of strain 94 rif-r	This study
Plasmids
p34S-Gm	Source of Gm^r-cassette	Dennis and Zylstra [20]
pEX18Tc	Gene replacement vector, Tet^r oriT sacB	Hoang et al. [21]
pAL-TA	Vector for cloning of PCR-products, Ap^r ori pUC Plac	Eurogen
pACYC184	Vector for cloning, ori p15 A, Tet^r Cam^r	Sambrook and Russel [22]
pUCP26-splIR	splI and splR genes cloned in pUCP26-host-range vector, Tc^r	Liu et al. [23]