A Novel Clinical Six-Flavoprotein-Gene Signature Predicts Prognosis in Esophageal Squamous Cell Carcinoma

Figure 2

Knockdown of the flavoprotein signature inhibits proliferation of ESCC cells. (a) siRNA-mediated knockdown of the flavoprotein signature was examined by using qRT-PCR. β-Actin served as the loading control. Negative control (NC) siRNA or siRNA targeting the flavoprotein signature (siRNA) was transfected into KYSE150 cells and KYSE510 cells. , , , Student’s t test. (b). Clone formation images and number of clones. Cell proliferation was determined in colony formation assays in which 2000 transfected cells were inoculated in each well of a six-well plate. Cultures were maintained for 2 weeks, and cells were then fixed, stained, and photographed. Each experiment was performed in triplicate, and results represent the mean ± SD of three experiments. , , , one-way ANOVA.

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