Inhibition of LOXL2 Enhances the Radiosensitivity of Castration-Resistant Prostate Cancer Cells Associated with the Reversal of the EMT Process

Figure 5

Salvage experiment confirmed that EMT process reversal dominated the mechanism for restoring radiosensitivity in DU145 cells after LOXL2 knockdown. (a) EMT phenotype was coercively restored by simultaneous E-cadherin knockdown in LOXL2-silenced DU145 cells, as represented by the restoration of the expression of vimentin and N-cadherin. (b, c) Coercively restoring the EMT process in LOXL2-silenced DU145 cells restored cellular radioresistance as determined by the clonogenic assay. (d-e) Coercively inducing the EMT process restored cellular radioresistance as determined by cell apoptosis study by Annexin V-FITC/PI double staining. denotes p <0.05 by Student's t-test.

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