Collagen/Gelatin Sponges (CGSs) Provide Both Protection and Release of bFGF: An In Vitro Study

Figure 3

The effect of the bFGF concentration on fibroblast proliferation. Proliferation of fibroblasts at (a)-(b) 140ng/mL, (c)-(d) 8.75 ng/mL, and (e)-(f) 35 ng/mL, in the absence of 2% FBS and (d) in the presence of FBS, respectively. CGS indicates that bFGF was added to CGS; L indicates that bFGF was added to small tubes and kept under the same conditions for the same amount of time. 2% FBS indicates that 2% FBS was added to the culture media; no FBS indicates that FBS was not added to the culture media. For samples in presence of FBS, control sample is P3, DMEM + 2% FBS. For samples in absence of FBS, control sample is Neg, DMEM. (N=6). The results are expressed as a ratio of the optical density (OD) values to the OD results of control samples P2. 1-way ANOVA, Tukey’s multiple comparisons test. p<0.001 and p<0.01.

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