Trans-Cinnamaldehyde Inhibits IL-1β-Stimulated Inflammation in Chondrocytes by Suppressing NF-κB and p38-JNK Pathways and Exerts Chondrocyte Protective Effects in a Rat Model of Osteoarthritis

Figure 5

TCA suppresses p38-JNK activation in IL-1β-stimulated SW1353 cells. The SW1353 cells were preprocessed with varying concentrations of TCA (2-20 μg/ml) for 2 h and then stimulated with IL-1β (10 ng/ml) for 6 h. The proteins were detected by Western blot. The protein expression of p-ERK (a), p-p38 (b), and p-JNK1/2 (c) was standardized based on the respective level of GAPDH protein. Value was expressed as relative changes in comparison to control, which was set to 1. P < 0.05.