Role of TGF-Beta1/SMAD2/3 Pathway in Retinal Outer Deep Vascular Plexus and Photoreceptor Damage in Rat 50/10 Oxygen-Induced Retinopathy
Figure 9
Rho expression and photoreceptor phenotype. The expression of Rho was presented in (a) with GAPDH as a loading control, and the quantification of Rho was displayed in (b). In western-blot, Rho in the Normoxia group was significantly higher than that in the OIR group, LY364947 decreased the expression in the OIR+LY364947 and Normoxia+LY364947 groups compared with their noninhibitor counterparts. (c) showed the representative images of photoreceptors at 1000×magnification (scale bar=10μm). The average sectional area and number of nucleus were calculated and plotted in the graphs (d, e). The average sectional area was significantly larger in the OIR group compared with the Normoxia group (P<0.05); similarly, OIR induction also increased the area in OIR+LY364947 group compared with the Normoxia+LY364947 group. OIR together with inhibitor administration further expanded the gap, as shown by the comparison of the OIR+LY364947 group with the Normoxia group. In terms of the number of nucleus (c), it was decreased by OIR induction, inhibitor application, and the two combined, in comparison with OIR, Normoxia+ LY364947, and OIR+ LY364947 with Normoxia group, respectively; inhibitor application also decreased the number in the OIR+LY364947 compared with its noninhibitor counterpart; furthermore, OIR induction reduced the nucleus number in comparison of the OIR versus Normoxia+ LY364947 group and OIR+ LY364947 versus Normoxia+ LY364947 group. Data were shown as means±SD. (P<0.05, P<0.01).