Enterocin BacFL31 from a Safety Enterococcus faecium FL31: Natural Preservative Agent Used Alone and in Combination with Aqueous Peel Onion (Allium cepa) Extract in Ground Beef Meat Storage
Table 2
Effect of BacFL31, APOE, and their combination on MetMb (%), protein carbonyl (nmoles carbonyl/mg protein), sulfhydryls (nmoles sulfhydryl/mg protein), peroxide values (meq peroxide/Kg of meat), TBARS (mg MDA/kg meat) and the conjugated dienes (µmol/mg of meat) of the ground beef meat during storage at 4°C.
Days of storage at 4°C
0
3
7
10
14
Protein oxidation products
MetMb
T0
23.98 ± 0.02
35.13 ± 0.04
40.9 ± 0.12
51.33 ± 0.15
58.23 ± 0.22
T1
24.03 ± 0.11
31.15 ± 0.13
34.93 ± 0.1b
37.14 ± 2.06c
43.06 ± 2.51
T2
23.85 ± 0.10
31.12 ± 0.09
36.09 ± 0.12
42.94 ± 0.19
45.03 ± 2.22
T3
23.93 ± 0.17
27.1 2± 0.11
34.26 ± 0.10
39.54 ± 0.21
42.2 ± 0.11
T4
23.61 ± 0.11
26.34 ± 0.12
32.04 ± 0.11
35.14 ± 0.13
40.23 ± 0.09
Carbonyls contents
T0
4.02 ± 0.9
5.11 ± 0.11
6.41 ± 0.78
5.84 ± 0.74
4.51 ± 0.69
T1
3.58 ± 0.11
4.3 ± 0.11
4.45 ± 0.08
3.85 ± 0.11
4.01 ± 0.15
T2
3.88 ± 0.11
4.5 ± 0.12
5.45 ± 0.18
4.1 ± 0.16
3.88 ± 0.13
T3
3.04 ± 0.16
3.55 ± 0.10
4.15 ± 0.48
3.1 ± 0.17
2.67 ± 0.10
T4
3.11 ± 0.33
2.22 ± 0.20
3.23 ± 0.37
3.38 ± 0.41
2.29 ± 0.33
Sulfhydryls groups
T0
45.88 ± 0.20
42.55 ± 0.19
39.14 ± 0.03
32.31 ± 1.11
29.14 ± 0.81
T1
45.45 ± 022
42.10 ± 2.10
40.6 ± 0.08
40.19 ± 0.81
37.60 ± 0.22
T2
46.03 ± 0.93
42.45 ± 0.01
39.07 ± 1.67
36.68 ± 1.61
33.55 ± 1.09
T3
45.53 ± 0.77
42.23 ± 1.01
43.07 ± 1.23
42.68 ± 1.22
40.55 ± 1.04
T4
46.07 ± 0.33
44.55 ± 1.06
43.27 ± 1.55
43.08 ± 0.28
42.19 ± 1.09
Lipid oxidation products
Peroxide values
T0
2.21 ± 0.51
6.32 ± 0.24
11.06 ± 0.41
14.22 ± 0.23
11.85 ± 0.82
T1
1.89 ± 0.57
4.74 ± 0.44
6.48 ± 0.11
7.9 ± 0.25
8.16 ± 0.64
T2
2.05 ± 0.45
5.05 ± 0.33
7.11 ± 0.25
9.48 ± 0.41
10.75 ± 0.32
T3
1.89 ± 0.17
3.95 ± 0.60
5.21 ± 0.11
7.58 ± 0.21
7.91 ± 0.45
T4
2.05 ± 0.22
4.11 ± 0.43
4.74 ± 0.31
7.58 ± 0.41
8.16 ± 0.32
TBARS value
T0
0.48 ± 0.12
1.45 ± 0.07
2.12 ± 0.09
2.71 ± 0.11
2.98 ± 0.12
T1
0.43 ± 0.01
0.81 ± 0.14
1.19 ± 0.13
1.58 ± 0.11
2.01 ± 0.12
T2
0.44 ± 0.12
1.09 ± 0.09
1.59 ± 0.12
2.01 ± 0.09
2.48 ± 0.08
T3
0.40 ± 0.07
0.8 ± 0.12
1.31 ± 0.13
1.61 ± 0.11
2.08 ± 0.13
T4
0.48 ± 0.11
0.69 ± 0.12
0. 9 ± 0.11
1.22 ± 0.13
1.88 ± 0.08
CD
T0
0.717 ± 0.13
0.752 ± 0.24
0.685 ± 0.13
0.629 ± 0.29
0.626 ± 0.23
T1
0.667 ± 0.65
0.689 ± 0.01
0.642 ± 0.09
0.616 ± 0.36
0.585 ± 0.21
T2
0.663 ± 0.33
0.681 ± 0.10
0.655 ± 0.14
0.613 ± 0.35
0.555 ± 0.23
T3
0.633 ± 0.14
0.686 ± 0.022
0.613 ± 0.11
0.525 ± 0.10
0.435 ± 0.13
T4
0.64 3± 0.22
0.681 ± 0.55
0.603 ± 0.33
0.505 ± 0.21
0.412 ± 0.22
±: standard deviation of three replicates. Values with a different letter (a - c) within a row of the same storage day of each treatment are significantly different (P < 0.05). Values with a different letter (A - E) within a column of the same treatment are significantly different (P < 0.05) by using Tukey’s post-hoc test.
