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BioMed Research International
Volume 2019, Article ID 4128315, 10 pages
https://doi.org/10.1155/2019/4128315
Research Article

Identification of Circular RNAs Regulating Islet β-Cell Autophagy in Type 2 Diabetes Mellitus

1Department of Vascular Thyroid Surgery, First Hospital Affiliated to Xinjiang Medical University, Urumqi 830054, China
2Department of Endocrinology, Second Hospital Affiliated to Xinjiang Medical University, Urumqi 830063, China
3Department of Endocrinology, Sanya Central Hospital, Sanya 572000, China

Correspondence should be addressed to Zongbao Li; moc.621@3299bzl and Li Zhang; moc.qq@6898351841

Received 23 May 2019; Revised 30 August 2019; Accepted 29 September 2019; Published 7 November 2019

Academic Editor: Ming D. Li

Copyright © 2019 Chao Bai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study is to identify the circular RNA (circRNA) expression profile that is functionally related to pancreatic islet β-cell autophagy and their potential regulation mechanisms in type 2 diabetes mellitus (T2DM). T2DM rat model was constructed by administration of high-fat and high-sugar diet. β-cells were isolated from islets by flow cytometry. CircRNA expression profile in β-cells was detected by circRNA microarrays, and the differentially expressed circRNAs were identified and validated by qRT-PCR. MicroRNA (miRNA) target prediction software and multiple bioinformatic approaches were used to construct a map of circRNA-miRNA interactions for the differentially expressed circRNAs. A total of 825 differentially expressed circular transcripts were identified in T2DM rats compared with control rats, among which 388 were upregulated and 437 were downregulated. Ten circRNAs were identified to have significant differences by qRT-PCR. GO analysis enriched terms such as organelle membrane and protein binding and the top enriched pathways for the circRNAs included MAPK signaling pathway. The differentially expressed circRNAs might involve in MAPK signaling pathway, apoptosis, and Ras signaling pathway. We speculate that these circRNAs, especially rno_circRNA_008565, can regulate the autophagy of islet β-cells via interactions with miRNA. Dysregulation of several circRNAs may play a role in T2DM development, and rno_circRNA_008565 may be a potential regulator of β-cell autophagy.