Copper (II) Ions Activate Ligand-Independent Receptor Tyrosine Kinase (RTK) Signaling Pathway

Figure 2

Copper (II) induced the RTK-mediated cellular signal pathway in a concentration-dependent manner. (a) A549 cells were treated with different concentrations of copper (II) ion for 10 min and the phosphorylation of EGFR, MET, AKT, and ERK was examined using western blotting. The phosphorylation level of EGFR (b), MET (c), ERK (d), and AKT (e) in each experiment was quantified and analyzed. Data are represented as mean ± S.D. of triplicate experiments (P<0.05, P<0.001).

(a)

(b)

(c)

(d)

(e)