Copper (II) Ions Activate Ligand-Independent Receptor Tyrosine Kinase (RTK) Signaling Pathway

Figure 5

Copper (II) promoted wound healing and proliferation. (a) Copper (II) ion enhanced wound healing. The A549 cells were treated with or without copper (II) ion (50 μM), and the wound-closure events were captured by a light microscope. The images were taken at 0 and 24 h. The black lines indicate boundaries between cells in the monolayer and the scratched areas uncovered by cells. Scale bar: 1000 μm. (b) Relative wound-closure rate was measured and analyzed. Data are presented as means ± S.D. (n = 5) (P<0.001). (c) A549 cells were treated with 50 μM copper (II) and the relative cell proliferation at 24 h and 48 h was determined using CCK8 assay. Data are presented as means ± S.D. (n = 5) (P<0.001).

(a)

(b)

(c)