A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

Table 1

Comparison of different expression platforms features. Developed based on [1, 7, 8].

Platform/host	Overall costs	Production time	Scale-up capacity	Propagation	Product yield	Product quality	Contamination risk	Purification cost
Transgenic plants	very low	medium	very high	easy	high	high	low	high
Plant cell culture	medium	medium	medium	easy	high	high	very low	medium
Plant viruses	low	low	high	feasible	very high	medium high	very low	high
Microalgae	low	high	high	easy	high	high	very low	medium
Yeast	medium	medium	high	easy	high	medium	low	medium
Bacteria	low	low	high	easy	medium	low	medium (e.g., endotoxins)	high
Mammalian cell culture	high	high	very low	hard	medium-high	high	very high (e.g., virus, prions, oncogenic DNA)	high
Transgenic animals	high	high	low	feasible	high	high	very high (e.g., virus, prions, oncogenic DNA)	high
Insect cell culture	medium	medium	high	feasible	high	medium	very low	medium
Filamentous fungi	low	high	high	easy	high	medium	low	low