(a) Total Callus Area (Cl.Ar). Total callus area (comprising newly formed bone, cartilage, and fibrous tissue within the ROI) increased with the increase of displacement amplitudes in the treatment groups. Largest Cl.Ar was found in the 0.4 mm group and the 0.8 mm group, smallest callus area in the control group. (b) New Bone Area (nB.Ar) within the total callus. Formation of new bone within the total callus area was lowest in the control group. Highest nB.Ar was found in the 0.4 mm group and the 0.8 mm group. (c) Cartilage Area (Cg.Ar) within the total callus. Presence of cartilage in the total callus area was very low in all groups. Variation within the groups was very high since cartilage was not present in all specimens. In the treatment groups Cg.Ar was slightly increased compared to the control group.