Rapid Enkephalin Delivery Using Exosomes to Promote Neurons Recovery in Ischemic Stroke by Inhibiting Neuronal p53/Caspase-3

Figure 1

Tar-exo-enkephalin synthesis, formulation, and physicochemical features. (a) Cryo-EM analysis showed the morphology of tar-exo-enkephalin. (b) Nanoparticle tracking analysis showed the size of tar-exo-enkephalin. (c) Western blot of CD63, HSP70, TSG101, and GAPDH in exosomes and MSC cells. (d) Immunofluorescence assay confirming the binding of transferrin to the tar-exo membrane. (e) The level of enkephalin quantified by ELISA into tar-exo after electroporation. (f) Changes in exosomes number of neuron (blue) after treatment with tar-exo (red) obtained by immunofluorescence. Scale bar, 20 μm (n=8 per group). Results are expressed as mean ± SD, and ##P< 0.01.

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