Improved Quality of Corn Silage When Combining Cellulose-Decomposing Bacteria and Lactobacillus buchneri during Silage Fermentation

Table 2

The change rule of cellulose-decomposing bacteria and heterolactic lactic acid bacteria on the fermentation characteristics of corn silage.

Day	Treatment	pH	WSC; %DM	LA; %DM	AA; %DM	LA: AA	Ethanol; %DM	NH3-N; %DM	NH3-N:TN
0d		5.33	22.01	0.61	0.20	3.05	0.12	0.019	0.016
2d	K	4.47	20.00	3.84	3.00	1.28	0.17	0.030	0.026
	Y	4.47	18.02	4.65	2.18	2.13	0.16	0.040	0.035
	YX	4.62	14.25	4.10	2.94	1.39	0.15	0.032	0.027
	SEM	0.03	0.71	0.06	0.39	-	0.005	0.003	-
4d	K	4.26	14.02	4.72	3.00	1.57	0.17	0.039	0.035
	Y	4.31	14.01	7.29	3.22	2.26	0.16	0.028	0.025
	YX	4.56	12.39	4.76	2.49	1.91	0.16	0.041	0.036
	SEM	0.02	0.70	0.17	0.10	-	0.003	0.003	-
6d	K	4.22	12.50	4.23	4.66	0.91	0.17	0.049	0.043
	Y	4.30	4.41	4.88	3.47	1.41	0.17	0.054	0.047
	YX	4.52	6.04	4.34	3.28	1.32	0.17	0.077	0.067
	SEM	0.01	0.29	0.10	0.36	-	0.001	0.004	-
8d	K	3.79	10.99	6.00	3.36	1.79	0.19	0.042	0.038
	Y	3.92	3.87	5.59	1.52	3.68	0.16	0.058	0.053
	YX	4.28	3.04	5.16	1.93	2.67	0.16	0.069	0.061
	SEM	0.02	0.26	0.35	0.32	-	0.011	0.003	-
10d	K	4.13	11.82	3.51	1.81	1.94	0.17	0.084	0.075
	Y	4.17	3.04	3.63	1.93	1.88	0.16	0.061	0.055
	YX	4.37	3.59	4.61	4.11	1.12	0.16	0.076	0.068
	SEM	0.004	0.32	0.33	0.35	-	0.002	0.003	-
15d	K	4.12	11.13	2.43	3.22	0.75	0.16	0.060	0.054
	Y	4.08	7.39	2.78	2.73	1.02	0.16	0.076	0.067
	YX	4.35	2.54	3.44	2.60	1.32	0.16	0.140	0.121
	SEM	0.01	0.49	0.06	0.17	-	0.002	0.007	-
25d	K	4.08	6.51	2.41	2.40	1.00	0.15	0.057	0.052
	Y	4.12	8.80	2.62	2.66	0.98	0.16	0.067	0.060
	YX	4.35	5.55	3.57	2.97	1.20	0.15	0.098	0.087
	SEM	0.01	0.49	0.09	0.22	-	0.004	0.003	-
35d	K	4.02	5.34	1.42	1.29	1.10	0.15	0.044	0.040
	Y	4.09	6.64	1.77	2.41	0.73	0.14	0.057	0.051
	YX	4.26	5.93	3.07	1.41	2.18	0.14	0.121	0.109
	SEM	0.004	0.25	0.05	0.28	-	0.006	0.004	-
45d	K	4.29	8.44	2.70	1.45	1.86	0.15	0.128	0.115
	Y	4.12	5.69	2.57	2.39	1.08	0.14	0.107	0.096
	YX	4.25	5.47	3.59	1.05	3.42	0.16	0.157	0.138
	SEM	0.02	0.33	0.19	0.42	-	0.006	0.005	-
60d	K	4.09	5.49	1.97	2.18	0.90	0.14	0.075	0.066
	Y	3.98	3.34	2.26	1.39	1.63	0.21	0.119	0.107
	YX	4.27	3.92	3.54	2.89	1.22	0.63	0.126	0.112
	SEM	0.02	0.17	0.11	0.25	-	0.092	0.009	-
P-value	M				NS	-	NS		-
	D					-			-
	M×D					-			-

Note: DM, dry matter; WSC, water soluble sugar; LA, lactic acid; AA, acetic acid; TN, total nitrogen; K, untreated corn silage with no inoculant applied; Y, heterolactic lactic acid bacteria; YX, heterolactic lactic acid bacteria and cellulose-decomposing bacteria; M, treatment; D, day; M×D, treatment × day; SEM, standard error of mean. Values followed by the different letters are significantly different at 5% level using Duncan test; , P < 0.05, , P < 0.01, NS, not significant.