Curcumin Induces Neural Differentiation of Human Pluripotent Embryonal Carcinoma Cells through the Activation of Autophagy

Figure 2

Curcumin induced apoptosis of NTERA2 cells. (a) MTT assay was performed to determine cell viability of NTERA2 cells. The percentage of NTERA2 cell viability after being treated with curcumin at various concentrations was presented, and the IC50 value was 12.75 μM. (b) The expression of apoptosis-related genes, P53, BAX, CAS9, CAS3, and BCL2, was determined by RT-PCR. (c) The relative expression of apoptosis-related genes was assessed by using GAPDH as the internal control. The values were presented as mean ± SD, n=3 ( < 0.05 versus control).

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