BioMed Research International / 2019 / Article / Tab 1 / Research Article
Curcumin Induces Neural Differentiation of Human Pluripotent Embryonal Carcinoma Cells through the Activation of Autophagy Table 1 The list of primer sets for PCR reaction of pluripotent genes, neurogenesis genes, apoptosis genes, autophagy-related genes, and ROS genes used in this study.
Primer Sequence (5 ′-3 ′) Pluripotent genes GAPDH (Forward)5′ACCTGACCTGCCGTCTAGAA3′ GAPDH (Reverse)5′TCCACCACCCTGTTGCTGTA3′ NANOG (Forward)5′AGCCTCTACTCTTCCTACCACC3′ NANOG (Reverse)5′TCCAAAGCAGCCTCCAAGTC3′ OCT4 (Forward)5′GACAACAATGAAAATCTTCAGGAGA3′ OCT4 (Reverse)5′TTCTGGCGCCGGTTACAGAACCA3′ Neurogenesis genes NeuroD1 (Forward)5′AGCCCTCTGACTGATTGCAC3′ NeuroD1 (Reverse)5′GTCTATGGGGATCTCGCAGC3′ TUJ1 (Forward)5′GCTCAGGGGCCTTTGGACATCTCTT3′ TUJ1 (Reverse)5′TTTTCACACTCCTTCCGCACCACATC3′ PAX6 (Forward)5′AACAGACACAGCCCTCACAAACA3′ PAX6 (Reverse)5′CGGGAACTTGAACTGGAACTGAC3′ Apoptosis genes CAS9 (Forward)5′AACAGGCAAGCAGCAAAGTT3′ CAS9 (Reverse)5′TCCATCTGTGCCGTAGACAG3′ CAS3 (Forward)5′TTTGTTTGTGTGCTTCTGAGCC3′ CAS3 (Reverse)5′ATTCTGTTGCCACCTTTCGG3′ BCL2 (Forward)5′CGCATCAGGAAGGCTAGAGT3′ BCL2 (Reverse)5′AGCTTCCAGACATTCGGAGA3′ BAX (Forward)5′AAGCTGAGCGAGTGTCTCAAGCGC3′ BAX (Reverse)5′TCCCGCCACAAAGATGGTCACG3′ P53 (Forward)5′CCCCTCCTGGCCCCTGTCATCTTC3′ P53 (Reverse)5′GCAGCGCCTCACAACCTCCGTCAT3′ Autophagy-related gene ATG5 (Forward)5′ACGCTGGTAACTGAC AAA G3′ ATG5 (Reverse)5′CACATGACATAA AGTGAGCC3′ ATG12 (Forward)5′GAGACACTCCCATAATGAA3′ ATG12 (Reverse)5′GTAGGACCAGTTTACCATC3′ LC3 (Forward)5′GATGTCCGACTTATTCGAGAGC3′ LC3 (Reverse)5′TTGAGCTGTAAGCGCCTTCTA3′ P62 (Forward)5′GCCATTAGGCAAGCTATGTG3′ P62 (Reverse)5′GGTGCAAGAAGCCATTTAGG3′ LAM1 (Forward)5′TCACCGTCGTGTTGTCCTTC3′ LAM1 (Reverse)5′ACCATCCAGGCGTACCTTTC3′ ROS genes SOD (Forward)5′CTAGCGAGTTATGGCGAC3′ SOD (Reverse)5′CATTGCCCAAGTCTCCAAC3′ GPX (Forward)5′CGCCAAGAACGAAGAGATTC3′ GPX (Reverse)5′CAACATCGTTGCGACACAC3′ CATALASE (Forward) 5′TCCGGGATCTTTTTAACGCCATTG3′ CATALASE (Reverse) 5′TCGAGCACGGTAGGGACAGTTCAC3′
