Sulfatases, in Particular Sulf1, Are Important for the Integrity of the Glomerular Filtration Barrier in Zebrafish

Figure 5

High dose but not low dose Vegfa 165 recombinant protein partially prevents eGFP-DBP loss from circulation in larvae but not in and larvae. Measurement of the maximum fluorescence intensity (AU) in the retinal vessel plexus at 96 hpf. Zebrafish embryos were injected in the yolk sac at the 1-2 cell stage with either a scrambled control (CTRL) or with sulf1 MO, sulf2a MO, or sulf2b MO and coinjected with Vegfa 165 protein. MO-induced , , and induced maximum fluorescence intensity reduction indicative of eGFP-DBP loss, coinjection of Vefga 165 protein 100 ng/μl partially prevented the fusion protein loss in larvae. The Vefga 165 protein 100 ng/μl coinjection in and larvae did not prevent eGFP-DBP loss.