VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

Figure 5

Quantification of EGFP intensity on cell monolayers. (a) HEK293, (b) HeLa, and (c) HK2 were incubated for 16 and 24 h with VLPs or mRNA. mRNA with lipofectamine and cells alone were used as positive and negative control of fluorescence, respectively. The best time for analysing the expression of EGFP was 24 h. The results are expressed as the relative fluorescence units (RFUs) in relation with the negative control.

(a)

(b)

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