VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

Figure 6

VLPs internalization in mammalian cells. HEK293 transfected with pVAX1-EGFP-DNA and mRNA with lipofectamine as controls in transmitted light and fluorescence microscopy using a 40X objective (a–d). HEK293 exposed to VLPs without lipofectamine in transmitted and fluorescence light taken with a 20X objective e and f, respectively. (g) HeLa and (h) HK2 cells exposed to VLPs show also fluorescence with a 10X objective.