Oxaliplatin Treatment Alters Systemic Immune Responses

Figure 2

Effects of oxaliplatin on the proportion of T cell populations and pro-/anti-inflammatory cytokines within the spleen. To determine any changes in the proportions of CD4⁺ and CD8⁺ T cells and Tregs we gated on CD3⁺CD4⁺/CD8⁺/GR-1^-/FOLR4⁺ events. Proinflammatory and anti-inflammatory cytokines were gated on IL-6, TNF-α, IL-10, and TGFβ events. Oxaliplatin treatment caused a significant increase in the proportion of CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T cells when compared to the vehicle-treated group (a, b). Conversely, oxaliplatin treatment caused a significant decrease in the proportion of CD3⁺ CD4⁺ FOLR4⁺ T cells when compared to the vehicle-treated group (c). No changes in IL-6 expression were observed between the vehicle-treated and the oxaliplatin-treated mice; however, a significant increase in TNF-α was noted in the oxaliplatin-treated group when compared to the vehicle-treated cohort (d). No changes in either anti-inflammatory cytokines were observed between the vehicle-treated and oxaliplatin-treated mice (e). Vehicle: n=5-9; oxaliplatin: n=5-14; P<0.01.

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