Oxaliplatin Treatment Alters Systemic Immune Responses

Figure 3

Effects of oxaliplatin on the proportion of activated T cell populations and B cells within the spleen. To determine any changes in the proportions of activated CD4⁺ and CD8⁺ T cells, we gated on CD4⁺ CD25⁺ CD69⁺, CD8⁺ CD25⁺ CD69⁺, and CD4⁺ FOLR4⁺ CD25⁺ CD69⁺ events. Oxaliplatin treatment did not cause any changes in the proportion of activated CD4⁺ T cells (a, b). However, a significant increase in the proportion of CD8⁺ CD69⁺, but not CD8⁺ CD25⁺ was observed following oxaliplatin treatment when compared to the vehicle-treated group (c, d). Oxaliplatin caused a significant increase in the proportion of CD4⁺ FOLR4⁺ CD69⁺, but not CD4⁺ FOLR4⁺ CD25⁺ Tregs, when compared to the vehicle-treated group (e, f). B cells were identified by gating on CD45⁺ TCRβ^- B220⁺ cells. Oxaliplatin treatment caused a significant reduction in the proportion of B cells when compared to the vehicle-treated cohort (g). Vehicle: n=5-9; oxaliplatin: n=5-14; P<0.01.

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