Oxaliplatin Treatment Alters Systemic Immune Responses

Table 1

Antibodies used for flow cytometry experiments in this study.

Cells	Primary antibody	Conjugate	Host species	Dilution
Pan-leukocyte marker	CD45	PerCP/Cy5.5	Mouse	1:400
Pan-T cell marker	CD3	Alexa Fluor 488	Mouse	1:400
T cell receptor	TCRβ	APC	Rat	1:250
Granulocytes	GR-1, CD11b	PE-Cy7	Rat	1:100
Cytotoxic T cells	CD8	Brilliant Violet 421	Rat	1:100
Helper T cells	CD4	Brilliant Violet 500	Rat	1:100
Regulatory T cells	Folate receptor 4	Alexa Fluor 647	Mouse	1:100
Activated T cells	CD25	PE-Cy7	Mouse	1:100
Activated T cells	CD69	APC-Cy7	Mouse	1:100
B cells	B220	FITC	Mouse	1:400
Macrophages	CD11b, Ly6C, Ly6G, CD206, F4/80	PE	Rat	1:200
Hematopoietic Stem and Progenitor Cell	CD34	FITC	Mouse	1:100
Hematopoietic Stem and Progenitor Cell	c-Kit	PE	Mouse	1:100
Hematopoietic Stem and Progenitor Cell	Sca-1	PE-Cy7	Mouse	1:100
Hematopoietic Stem and Progenitor Cell	Lineage cocktail	APC	Mouse	1:100
Cytokines
IL-6	IL-6	APC	Mouse	1:100
TNF-	TNF-α	Brilliant Violet™ 510	Mouse	1:100
IL-10	IL-10	APC	Mouse	1:100
TGF	TGFβ	Brilliant Violet™ 421	Mouse	1:100