High Expression of Acid-Sensing Ion Channel 2 (ASIC2) in Bone Cells in Osteoporotic Vertebral Fractures

Figure 5

Inhibition of ASICs activities by amiloride in an acidic environment. A 0.2mM amiloride (ASIC antagonist) was added into the osteogenic medium culture (pH of 6.9, 7.4, 8.0) on the 7 days and incubation 3hr, 6hr, and 12hr to analyze the differences of Na⁺/K⁺ ATPase change. Normally, Na⁺/K⁺ ATPase RNA content was significantly increased in the pH 6.9 cultured osteoblasts. While amiloride was added into the osteogenic medium, it caused a significant decrease in Na⁺/K⁺ ATPase in the pH 6.9 cultured osteoblasts. p<0.05.