High Expression of Acid-Sensing Ion Channel 2 (ASIC2) in Bone Cells in Osteoporotic Vertebral Fractures

Table 1

Primer sequences for relative quantification of ASIC transcripts.

Gene	Forward primer	Reverse primer	Accession
ASIC1	CCCTGTTGTCTTGGTGAC	ATGGTGAGGTAGGATGTT	NM 020039
ASIC2	CGCCACTTCGAGGGAATCAG	GTAAACACGGAGGAGAAGTTGTG	NM 183377
ASIC3	GGTGTTCCGAGACAAGGTCC	GGTGGGAGGTCAGATTGGTG	NM 020322
ASIC4	GGTAGACATCCTCAACCGCA	GCGAGTATAGACCACAGAGAAGT	NM 182847
ALP	ACCATTCCCACGTCTTCACATTTG	AGACATTCTCTCGTTCACCGCC	NM 001127501
OPN	AGCCAGGACTCCATTGACTCGAAC	GTTTCAGCACTCTGGTCATCCAGC	NM 001040058
OC	ATGAGAGCCCTCACACTCCTC	GCCGTAGAAGCGCCGATAGGC	NM 199173
Na^+/K^+ ATPase	TGTCCAGAATTGCAGGTCTTTG	ACAACTGGTACTTGTTGGTGGA	NM 000701
GAPDH	GTCGGAGTCAACGGATTT	CAACAATATCCACTTTACCAGAG	NM 002046

Gene bank accession number of cDNA available on line at http://www.ncbi.nlm.nih.gov/;
ASIC: acid-sensing ion channel; ALP: alkaline phosphatase; OPN: osteopontin; OC: osteocalcin; GAPDH: glyceraldehyde-3-phosphate dehydrogenase