Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion

Figure 3

Structural representation of the conformational changes of PBPs. The figure shows a periplasmic maltose-binding protein (MBP) from E. coli in its open apo-form (PDB accession 1OMP) and in its closed holo-form upon maltose binding (PDB accession 1ANF). The flexibility of the hinge region allows the large conformation change in MBP. Red and green circles indicate regions which are separated in the open form and are proximate in the closed form. The blue circle shows a region in which packing is changed between open and close forms. Structural representation was rendered in PyMol.