N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor

Figure 5

NAC attenuates C5a/C5aR deposition in renal parenchyma. (a) Immunohistochemistry (IHC) showed that the C5a expression was in the CN, Cis, Cis+NAC, and NAC group. The C5a expression was increased in the Cis group and decreased after NAC treatment in the Cis+NAC group; (b and c) Real-time PCR of C5a and C5aR was performed to evaluate the expression level in each group in kidney. (d) the protein level of C5aR in kidney was detected by Western blot. (e) the histogram shows the relative intensity for each marker normalized to GAPDH. The values are presented as the means±SEM, n = 8. P < 0.05, P < 0.01, and P < 0.001.

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