LC-MS/MS Analysis and Hepatoprotective Activity of Artichoke (Cynara scolymus L.) Leaves Extract against High Fat Diet-Induced Obesity in Rats
Table 1
Identification of phenolic compounds in ethanol extract from leaves of C. scolymus using their retention times, LC/MS, and LC–MS/MS data.
Peak
RT (min)
m/z
Mode (+/-)
(min)
MS/MS
Tentative ID
1
3.00
122.3
+
200-700
82
Salicylic acid-o-hexoside
2
3.7
117.2
-
200-700
100
Chlorogenic acid
3
4.2
112
-
200-700
214.8, 178.8, 352.8
3-mono-o-cafeoylquinic acid
4
5.3
141
-
200-700
265.9
Apigenin-7-glucoside
5
8.4
223
-
200-700
331.4
Unknown
6
10.30
275
-
200-700
529.7, 350.8, 284.7
Luteolin-7-o-rutinoside
7
13.5
327.1
-
200-700
170.9
Dihydroxypropiophend-hexoside
8
14.8
395
-
200-700
529.7, 288. 9,307
Kampferol 3-o-rutinoside
9
16.2
431
-
200-700
434.7, 294.9, 344.8
Quercetin-o-pentoside
10
23.3
623
-
200-700
255.1
Caffeic acid
Identification was aided by comparison with reference standards that were available and by correlation with previous literature reports. Peak numbers and retention times (Rt) refer to LC/MS chromatograms in Figure 2.
