Research Article

Ester-Producing Mechanism of Ethanol O-acyltransferase EHT1 Gene in Pichia pastoris from Shanxi Aged Vinegar

Figure 4

qRT-PCR for quantification of the expression level of EHT1 in KO and OE. WT was used as the reference control. Three lines of OE (OE-1, OE-2, OE-3), one line of KO, and one line of WT were tested. Each line represents triplication. P. pastoris actin was used as the reference gene.