Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2

Figure 7

EBF2 is directly targeted by miR-182-3p. (a) Sequences of CDK6 3′-UTR and its mutant type. (b) 293T cells were cotransfected with an EGFP reporter plasmid (containing 3′UTR of EBF2 or its mutant type), either alone or in combination with a miR-182-3p mimic. EGFP levels were measured after 48 hours using fluorescence spectrophotometer. (c) Fluorescence intensity of all groups (n=3, <0.05).

(a)

(b)

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