In Vivo, Proteomic, and In Silico Investigation of Sapodilla for Therapeutic Potential in Gastrointestinal Disorders

Figure 6

Effects of Mz.Aq extract on ethanol-mediated stomach histopathologic changes. Stomach tissues (n = 5) from each experimental group were processed for histological evaluation at 1 hour after ethanol challenge. (a) Representative histological changes of apoptotic markers (TNF-α, P-NFκB, and COX-2) in stomach, scale bar = 20 μm, magnification 40x: control group, ethanol group, Mz.Aq extract group, and omeprazole group. (b, c) Severity scores of stomach injury in different groups (n = 5) calculated via relative density of value (AU) and relative integrated density. Data were analyzed by two-way ANOVA followed by Tukey’s post hoc test using GraphPad Prism software. Mean ± SEM. versus ethanol group; versus ethanol group. (d) Histopathological examination of saline group, ethanol group, test group, and omeprazole group.

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