Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models

Figure 1

Immunophenotyping and biomass quantification of tumor cells. (a) Multicolor flow cytometry was conducted on a BD FACS Verse™ to examine the abundance of immune-related molecules as stated in material and methods. (b) Quantitative analyses of cell vitality using 0.2% crystal violet staining after 72 h incubation with test substances. Biomass reduction (%) after treatment was quantified by normalization to control values (untreated cells, set to be 100 %). N = 3 independent experiments, mean ± SD, p < 0.01; p < 0.05 versus control. Unpaired Student’s t-test. CIN, chromosomal instable; CIMP, CpG island methylator phenotype; MSI, microsatellite instability/instable; PD-L1/PD-L2, programmed cell death 1 ligand 1/2; HROC, Hansestadt Rostock Colon.

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