Calcium Fluxes in Work-Related Muscle Disorder: Implications from a Rat Model

Figure 5

Western blot analysis of muscles. Muscle samples from each limb and muscle subgroup of each rat were analyzed using SDS-Page and western blot analysis (each replicated at least 5 times). Full-length blots are shown for the proteins of interest (pCam, RyR1, Casq1, and SERCA1). Membranes were cut into upper and lower halves and one-half stained for GAPDH (the loading control), and the other for the protein of interest, as shown clearly in (c) and (d). Upper half of the membrane shown in panel C was stripped and reprobed for SERCA (panel D). (a) Representative western blot showing pCam and GAPDH stained bands. pCam showed significantly increased expression levels in the Ext and Flex muscles of HRHF-RL, compared to FRC-R limbs. (b) RyR significantly increased the expression levels in the Ext and Flex muscles of HRHF-RL, compared to FRC-R limbs. (c) Casq1 differed significantly with increased expression levels in the Ext muscles of HRHF-RL, compared to FRC-R limbs. (d) SERCA1 differed significantly with increased expression levels in the Ext muscles of HRHF-RL, compared to FRC-R limbs. and , compared to FRC-R. The order of muscle loaded into individual wells of gels differed across analytes assayed; however, the order is consistently presented in the graphs. ANOVA results are shown in individual panels as mean ± SEM.

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