High Glucose Enhances the Odonto/Osteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway

Figure 3

Effects of high glucose on the odonto/osteogenic differentiation of SCAPs. (a, b) The expression of odonto/osteogenic genes (ALP, RUNX2, OSX, OCN, and DSPP) in 25 mmol/L high glucose-treated SCAPs was significantly higher than that in 5 mmol/L group at days 3 and 7. GAPDH served as an internal control. Values were presented as means± SD, n = 6. P < 0.01. (c) Western blot showed the odonto/osteogenic proteins (e.g., ALP, RUNX2, OSX, OCN, and DSP) in 25 mmol/L group were significantly higher than those in 5 mmol/L group at days 3 and 7. ACTIN served as an internal control. (d) Grayscale analysis of panel C. P < 0.05; P < 0.01.

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