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Figure 4: NF-κB pathway involvement in high glucose-mediated odonto/osteogenic differentiation of SCAPs. (a) The protein levels of P65, p-P65, IκBα, and p-IκBα in the cytoplasm of 25 mmol/L high glucose-treated SCAPs at 0, 15, 30, and 60 min, respectively. β-ACTIN served as an internal control. The nuclear P65 expression of 25 mmol/L high glucose-treated SCAPs at 0, 15, 30, and 60 min, respectively. Histone 3 served as an internal control. (b) Quantitative analysis for the ratios of p-IκBα/IκBα and p-P65/P65 and grayscale analysis of panel A after 25 mmol/L glucose treatment at indicated time points. Values were presented as means± SD, n = 6. P< 0.05; P< 0.01. (c) The expression of odonto/osteogenic genes (ALP, RUNX2, OSX, OCN, and DSPP) in 25 mmol/L+BMS345541 group was obviously decreased as compared with that in 25 mmol/L group. Values were presented as means ± SD, ≥ 2, P< 0.01; 1 < < 2, P < 0.05, n=3.