Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System

Figure 10

Cell cycle synchronization affects cell fate through MAPK signaling pathway. (a-b) BrdU and Ki-67 staining before and after cell cycle synchronization (BrdU⁺ is red and Ki67 is green). (c) Quantitative analysis of BMSC numbers at the G0 stage. Data represent the means ± SE; P <0.01. (d) Expression of the bFGF receptor FGFR1 at different time points for synchronized and nonsynchronized cells. S indicates the synchronized group and NS indicates the nonsynchronized group. (e) The expression of the c-fos transcription factor in the MAPK signaling pathway for synchronized and nonsynchronized cells. (f) The expression of the ERK transcription factor in the MAPK signaling pathway for synchronized and nonsynchronized cells. (g) The expression difference of the molecular switch BTG2, which regulates the transformation from cell proliferation to differentiation, before and after cell cycle synchronization. (h) The expression of BTG2 for the group of soluble bFGF and condition medium. Data represent the means ± SE.

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