Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Lineage Cells Induced by bFGF-Chitosan Controlled Release System

Figure 2

Morphological observation of P3 BMSCs and characterization by flow cytometry and immunofluorescence. (a) Morphological observation of P3 BMSCs. The cells were fibroblast-like shaped and possessed a screw-like alignment. (b-c) Immunofluorescent staining of CD34 and CD31, respectively. (d) Expression of CD90 and CD45, respectively, in P3 BMSCs by flow cytometry. Upper-left: CD90⁺/CD45^- cells comprised approximately 94.12% of the total cells. (e) Expression of CD90 and CD34 in P3 BMSCs by flow cytometry. Upper-left: CD90⁺/CD34^- cells comprised approximately 94.83% of the total cells. (f) Expression of CD90 and CD31 in P3 BMSCs by flow cytometry. Upper-left: CD90⁺/CD31^- cells comprised approximately 94.38% of the total cells. (g) Expression of CD105 and CD45 in P3 BMSCs by flow cytometry. Upper-left: CD105⁺/CD45^- cells comprised approximately 80.47% of the total cells. (h) Expression of CD73 and CD45 in P3 BMSCs by flow cytometry. Upper-left: CD73⁺/CD45^- cells comprised approximately 95.59% of the total cells.

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