mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells

Figure 2

AZD8055 inhibits mTORC1 and mTORC2 activation simultaneously and decreases the accumulation of TAG, PA, and DHA in the culture medium. (a) Cells were treated with 100 nM AZD8055 for 12 h or 24 h and the content of TAG (p=0.031, p=0.001), PA (p=0.0001, p=0.0002), and DHA (p=0.335, p=0.006) was measured by ELISA. AZD8055 significantly decreased the accumulation of TAG, PA, and DHA in the culture medium. (b) AZD8055 attenuates the phosphorylation of downstream targets of mTORC1, including S6 (Ser240/244), and 4EBP1 (Thr37/46). (c) AZD8055 attenuates the phosphorylation of mTOR (Ser2448) and downstream target of mTORC2, AKT (Ser 473). p<0.05, p<0.01, ns, no significantly; n=3 biological replicates. Error bars indicate SD.

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