mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells

Figure 4

RICTOR silencing decreases mTORC2 activation but not mTORC1 in pBMECs. (a) RICTOR mRNA level is decreased in primary bovine mammary epithelial cells transfected with pRNAT-U6.1/Neo-RICTOR-shRNA (p=0.01). (b) RICTOR protein level is decreased in primary bovine mammary epithelial cells transfected with pRNAT-U6.1/Neo-RICTOR-shRNA. (c) RICTOR silencing attenuates the phosphorylation of mTOR (Ser2448) and downstream target of mTORC2, AKT (Ser 473). (d) RICTOR silencing does not attenuate the phosphorylation of downstream targets of mTORC1, including S6 (Ser240/244), and 4EBP1 (Thr37/46). p<0.05, p<0.01, ns, no significantly; n=3 biological replicates. Error bars indicate SD.

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