mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells

Table 1

The target genes and primers for qPCR.

Gene Name	Primer sequence (5′→3′)
RICTOR (NM_001144096.3)	P1:CCCGACAGTATGTACGAGC
	P2: ATCCCAGAGTTTCCAGGTT
PPARG (NM_181024.2)	P1:GACGGGAAAGACGACAGACAAATC
	P2: CGTTCAAGTCAAGGTTCACAAAG
DGAT1 (XM_025001413.1)	P1:AAGCCCTTCAAGGACATG
	P2:GCAGTAGAAGAAGATGAGCC
LPIN1 (NM_001206156.2)	P1:AAAGTGAGCCAAAACGGGT
	P2:TTCTTTTCGATCACTTCCCTG
ACACA (NM_174224.2)	P1:ATCCCACGCATCTATGTAG
	P2:AGCACTGACTCTCTTGTAATC
FASN (NM_001012669.1)	P1:GGACGCTTTCCGTTACAT
	P2:CCAGTGATGATGTAGCTCTTG
ACTB (NM_173979.3)	P1:CACCACGGCCGAGCGGGAAATC
	P2:AGAGCCTCAGGGCAGCGGAACC